Molecular Characterization of Salmonella enterica Serovar Typhimurium Isolated from Human, Food, and Animal Sources in Malaysia

Ngoi, Soo Tein and Lindstedt, Bjørn-Arne and Watanabe, Haruo and Thong, Kwai Lin (2013) Molecular Characterization of Salmonella enterica Serovar Typhimurium Isolated from Human, Food, and Animal Sources in Malaysia. Japanese Journal of Infectious Diseases, 66 (3). pp. 180-188. ISSN 1344-6304, DOI https://doi.org/10.7883/yoken.66.180.

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Official URL: https://doi.org/10.7883/yoken.66.180

Abstract

Salmonella Typhimurium is an important nontyphoidal Salmonella serovar associated with foodborne diseases in many parts of the world. This organism is the major causative agent of nontyphoidal salmonellosis in Malaysia. We aimed to investigate the genetic profiles of the strains isolated from clinical, zoonotic, and dietary sources in Malaysia using multilocus variable number tandem repeat analysis (MLVA) and pulsed-field gel electrophoresis (PFGE). By focusing on the 5 common variable number tandem repeat (VNTR) loci, we found that PFGE (D = 0.99) was more discriminative than MLVA (D = 0.76). The low MLVA score might be because of a lack of VNTR loci STTR6 (81.0%) and STTR10pl (76.2%). Both subtyping methods suggested that our S. Typhimurium strains were largely endemic with limited genetic variation. Furthermore, we observed that biphasic S. Typhimurium strains were dominant (99%) and multidrug resistance was prevalent (50%) within our sample pool. The most frequently observed phenotypes were resistance to compound sulfonamides (49%), tetracycline (51%), and streptomycin (52%). In this study, we documented the genetic relationship, antimicrobial resistance characteristics, and flagellar-phase dominance among S. Typhimurium strains found in Malaysia.

Item Type: Article
Funders: UNSPECIFIED
Uncontrolled Keywords: antimicrobial resistance; flagellar phase; genotyping; molecular characterization; Salmonella Typhimurium
Subjects: Q Science > QR Microbiology
Divisions: Faculty of Science > Institute of Biological Sciences
Depositing User: Prof. Dr Kwai Lin Thong
Date Deposited: 25 Jun 2014 06:55
Last Modified: 19 Nov 2019 08:18
URI: http://eprints.um.edu.my/id/eprint/10645

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