Tan, W.C. and Jaganath, I.B. and Manikam, R. and Sekaran, S.D. (2013) Evaluation of antiviral activities of four local malaysian phyllanthus species against herpes simplex viruses and possible antiviral target. International Journal of Medical Sciences, 10 (13). pp. 1817-1829. ISSN 1449-1907,
Full text not available from this repository.Abstract
Nucleoside analogues such as acyclovir are effective antiviral drugs against herpes simplex virus infections since its introduction. However, with the emergence of acyclovir-resistant HSV strains particularly in immunocompromised patients, there is a need to develop an alternative antiherpetic drug and plants could be the potential lead. In this study, the antiviral activity of the aqueous extract of four Phyllanthus species were evaluated against herpes simplex virus type-1 (HSV-1) and HSV-2 in Vero cells by quantitative PCR. The protein expressions of untreated and treated infected Vero cells were studied by 2D-gel electrophoresis and Western blot. This is the first study that reported the antiviral activity of P. watsonii. P. urinaria was shown to demonstrate the strongest antiviral activity against HSV-1 and HSV-2, with SI > 33.6. Time-of-addition studies suggested that the extract may act against the early infection stage and the replication stage. Protein expression studies indicated that cellular proteins that are involved in maintaining cytoskeletal structure could be potential target for development of antiviral drugs. Preliminary findings indicated that P. urinaria demonstrated potent inhibitory activity against HSV. Hence, further studies such as in vivo evaluation are required for the development of effective antiherpetic drug.
| Item Type: | Article |
|---|---|
| Funders: | UNSPECIFIED |
| Additional Information: | ISI Document Delivery No.: 268IK Times Cited: 0 Cited Reference Count: 38 Tan, Wee Chee Jaganath, Indu Bala Manikam, Rishya Sekaran, Shamala Devi Malaysian Agriculture and Research Development Institute Grant 53-02-03-1002; University of Malaya IPPP student research grant PS181/2010A, PV053/2011b This study was supported by the Malaysian Agriculture and Research Development Institute Grant (53-02-03-1002) and the University of Malaya IPPP student research grant (PS181/2010A and PV053/2011b). We would like to acknowledge the Medical Biotechnology Laboratory, Faculty of Medicine, University of Malaya for their permission to use the 2D-GE equipment and for providing the mass spectrometry analysis in this study. Ivyspring int publ Lake haven |
| Uncontrolled Keywords: | Herpes simplex virus Phyllanthus antiviral cytoskeleton medicinal-plants type-2 infection alpha-actinin in-vitro hsv-2 amarus resistance acyclovir proteins extracts |
| Subjects: | R Medicine |
| Divisions: | Faculty of Medicine |
| Depositing User: | Ms azrahani halim |
| Date Deposited: | 20 Aug 2014 01:22 |
| Last Modified: | 20 Aug 2014 01:22 |
| URI: | http://eprints.um.edu.my/id/eprint/10625 |
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