Acute toxicity and gastroprotection studies of a newSchiff base derived copper (II) complex against ethanol-induced acute gastric lesions in rats

Abdulla, M.A. and Hajrezaie, M. and Golbabapour, S. and Hassandarvish, P. and Gwaram, N.S. and Hadi, A.H.A. and Mohd Ali, H. and Majid, N. (2012) Acute toxicity and gastroprotection studies of a newSchiff base derived copper (II) complex against ethanol-induced acute gastric lesions in rats. PLoS ONE. ISSN 1932-6203

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Abstract

Background: Copper is an essential element in various metabolisms. The investigation was carried out to evaluate Acute gastroprotective effects of the Copper (II) complex against ethanol-induced superficial hemorrhagic mucosal lesions in rats. Methodology/Principal Findings: Rats were divided into 7 groups. Groups 1 and 2 were orally administered with Tween 20 (10% v/v). Group 3 was orally administered with 20 mg/kg omeprazole (10% Tween 20). Groups 4-7 received 10, 20, 40, and 80 mg/kg of the complex (10% Tween 20), respectively. Tween 20 (10% v/v) was given orally to group 1 and absolute ethanol was given orally to groups 2-7, respectively. Rats were sacrificed after 1 h. Group 2 exhibited severe superficial hemorrhagic mucosal lesions. Gastric wall mucus was significantly preserved by the pre-treatment complex. The results showed a significant increase in glutathione (GSH), superoxide dismutase (SOD), nitric oxide (NO), and Prostaglandin E2 (PGE(2)) activities and a decrease in malondialdehyde (MDA) level. Histology showed marked reduction of hemorrhagic mucosal lesions in groups 4-7. Immunohistochemical staining showed up-regulation of Hsp70 and down-regulation of Bax proteins. PAS staining of groups 4-7 showed intense stain uptake of gastric mucosa. The Acute Toxicity revealed the non-toxic nature of the compound. Conclusion/Significance: The gastroprotective effect of the Copper (II) complex may possibly be due to preservation of gastric wall mucus; increase in PGE(2) synthesis; GSH, SOD, and NO up-regulation of Hsp70 protein; decrease in MDA level; and down-regulation of Bax protein.

Item Type: Article
Uncontrolled Keywords: Copper; Rat; Acute gastroprotective; Ethanol-induced superficial hemorrhagic mucosal lesions.
Subjects: R Medicine > R Medicine (General)
Divisions: Faculty of Medicine
Depositing User: Ms. Suhaila Syakila Alby
Date Deposited: 18 Jun 2014 01:02
Last Modified: 18 Jun 2014 01:02
URI: http://eprints.um.edu.my/id/eprint/10582

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