Molecular characterization and comparative sequence analysis of defense-related gene, oryza rufipogon receptor-like protein kinase 1

Law, Y.S. and Gudimella, R. and Song, B.K. and Ratnam, W. and Harikrishna, J.A. (2012) Molecular characterization and comparative sequence analysis of defense-related gene, oryza rufipogon receptor-like protein kinase 1. International Journal of Molecular Sciences. pp. 9343-9362. ISSN 1422-0067

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Abstract

Many of the plant leucine rich repeat receptor-like kinases (LRR-RLKs) have been found to regulate signaling during plant defense processes. In this study, we selected and sequenced an LRR-RLK gene, designated as Oryza rufipogon receptor-like protein kinase 1 (OrufRPK1), located within yield QTL yld1.1 from the wild rice Oryza rufipogon (accession IRGC105491). A 2055 bp coding region and two exons were identified. Southern blotting determined OrufRPK1 to be a single copy gene. Sequence comparison with cultivated rice orthologs (OsI219RPK1, OsI9311RPK1 and OsJNipponRPK1, respectively derived from O. sativa ssp. indica cv. MR219, O. sativa ssp. indica cv. 9311 and O. sativa ssp. japonica cv. Nipponbare) revealed the presence of 12 single nucleotide polymorphisms (SNPs) with five non-synonymous substitutions, and 23 insertion/deletion sites. The biological role of the OrufRPK1 as a defense related LRR-RLK is proposed on the basis of cDNA sequence characterization, domain subfamily classification, structural prediction of extra cellular domains, cluster analysis and comparative gene expression.

Item Type: Article
Additional Information: Institute of Biological Sciences, University of Malaya
Uncontrolled Keywords: Oryza rufipogon; RPK1; LRR domain; kinase domain; disease resistance
Subjects: Q Science > Q Science (General)
Divisions: Faculty of Science
Depositing User: Ms. Nur Azzura Azzahra Jaafar
Date Deposited: 15 Oct 2014 00:53
Last Modified: 15 Oct 2014 00:53
URI: http://eprints.um.edu.my/id/eprint/10452

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