Sponges of Tioman and their actinomycetes inhabitants

Sabaratnam, Vikineswary and Christabel, L.J. and Thong, Kwai Lin and Tan, Geok Yuan Annie and Affendi, Y.A. (2008) Sponges of Tioman and their actinomycetes inhabitants. In: Natural history of the Pulau Tioman Group of Islands. IOES monograph series . University of Malaya, Kuala Lumpur, pp. 35-41. ISBN 9789839576351

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Abstract

Actinomycetes were isolated from marine sponges of Tioman Island and taxonomically characterized up to genus level. Four hundred and seven putative actinomycetes were recovered from39 marine sponge samples collected from nine sites in Tioman Island using standard isolation protocols developed in the study. The actinomycetes population ranged from 2.72 x 102 to 0.6 x 102 colony forming unit (cfu)/g of dry sample. Isolates were then assigned into 11 color groups. Fifty three morphologically different representatives observed under the binocular light microscope were selected for chemotaxonomic, cultural, morphological and physiological characteristization. Chemotaxonomically, 21 (41%) isolates with L,L-diaminopimelic acid (DAP) and no diagnostic sugars pattern in the whole-cell wall hydrolysate were tentative streptomycetes (chemotype I), while 32 (59%) isolates with meso-DAP with observed three different sugars pattern were tentative non-streptomycetes (chemotype II/D, IV/A and III/B). Based on salt tolerance and carbon utilization test, forty five halo-groups and forty one carbo-groups were observed, indicating probable forty one to forty five different species or strains. The tentative genus diversity of rare actinomycetes in the marine sponges was Actinoplanes, Micromonospora, Sprilliplanes, Pseudonocardia, Nocardia, Rhodococcus, Saccharomonospora and Salinispora. Thirty eight crude extracts were tested against 13 bacterial strains and three fungal strains. Thirty four (64.2%) isolates exhibited antibacterial and antifungal activities, out of which eight (18.9%) isolates exhibited broad spectrum bioactivity.

Item Type: Book Section
Uncontrolled Keywords: Natural history; Malaysia; Tioman Island (Pahang)
Subjects: Q Science > Q Science (General)
Q Science > QH Natural history
Q Science > QR Microbiology
Divisions: Faculty of Science > Institute of Biological Sciences
Depositing User: Prof. Dr Kwai Lin Thong
Date Deposited: 04 Nov 2014 03:00
Last Modified: 08 Nov 2019 07:34
URI: http://eprints.um.edu.my/id/eprint/10351

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