Protein markers useful in authenticating eurycoma longifolia contained herbal aphrodisiac products

Vejayan, J. and Iman, V. and Foong, S.L. and Ibrahim, H. (2013) Protein markers useful in authenticating eurycoma longifolia contained herbal aphrodisiac products. Malaysian Journal of Science, 32 (1). pp. 15-23. ISSN 1394-3065,

[img]
Preview
 PDF
PROTEIN_MARKERS_USEFUL_IN_AUTHENTICATING_Eurycoma_longifolia_CONTAINED_HERBAL_APHRODISIAC_PRODUCTS.pdf - Published Version
Download (386kB)

Abstract

Eurycoma longifolia has been widely recognized for its aphrodisiac benefits and many herbal products incorporated with its extract been sold worldwide. The products in various forms of capsules or spherical tablets were appropriately solubilized in water and their protein content were separated using two dimensional electrophoresis (IPG strip of pI 3-10; 15% homogenous gel) and stained in coomassie blue. In this study using a standardized extract known for its biological activity, CFPR-JV1, two distinctive protein markers were detected within 2DE gel. The markers were spots found approximately at molecular weight of 14kDa at the basic extremities. The markers were not detected in a selected herbal product devoid of Eurycoma longifolia extract. In screening markers on a total of 23 National Pharmaceutical Control Bureau (NPCN) approved and 6 NPCB un-approved products claimed to have included Eurycoma longifolia extracts gave results with 18 of the 29 (approximately 62%) products observed with the presence of at least marker A, while the rest did not show presence of any markers. This enabled authentication of the products tested related to its active constituent. It is expected that the protein markers in addition to the existing chemical marker, Eurycomanone, may serve to be useful in authenticating genuine, unadulterated or sufficiently enough amount of Eurycoma longifolia in herbal aphrodisiac products.

Item Type: Article
Funders: UNSPECIFIED
Additional Information: Institute of Biological Sciences, University of Malaya
Uncontrolled Keywords: Plant; aphrodisiac; standardization; two dimensional electrophoresi; protein
Subjects: Q Science > Q Science (General)
Divisions: Faculty of Science > Institute of Biological Sciences
Depositing User: Ms. Jamilah Salleh
Date Deposited: 27 Oct 2014 01:42
Last Modified: 27 Apr 2021 07:56
URI: http://eprints.um.edu.my/id/eprint/10134

Actions (login required)

View Item View Item
UM Research Repository is powered by EPrints 3 which is developed by the School of Electronics and Computer Science at the University of Southampton. More information and software credits.